{"publication_identifier":{"issn":["0022-2836"]},"intvolume":"       305","publication":"Journal of Molecular Biology","date_created":"2025-01-23T15:14:52Z","date_updated":"2025-01-27T10:02:20Z","user_id":"220548","page":"523-533","author":[{"id":"202382","first_name":"Anke","full_name":"Rattenholl, Anke","last_name":"Rattenholl","orcid_put_code_url":"https://api.orcid.org/v2.0/0009-0003-6828-5778/work/176705810","orcid":"0009-0003-6828-5778"},{"last_name":"Ruoppolo","full_name":"Ruoppolo, Margherita","first_name":"Margherita"},{"first_name":"Angela","last_name":"Flagiello","full_name":"Flagiello, Angela"},{"first_name":"Maria","last_name":"Monti","full_name":"Monti, Maria"},{"full_name":"Vinci, Floriana","last_name":"Vinci","first_name":"Floriana"},{"first_name":"Gennaro","last_name":"Marino","full_name":"Marino, Gennaro"},{"first_name":"Hauke","last_name":"Lilie","full_name":"Lilie, Hauke"},{"last_name":"Schwarz","full_name":"Schwarz, Elisabeth","first_name":"Elisabeth"},{"first_name":"Rainer","last_name":"Rudolph","full_name":"Rudolph, Rainer"}],"doi":"10.1006/jmbi.2000.4295","publisher":"Elsevier BV","issue":"3","_id":"5380","status":"public","title":"Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor","type":"journal_article","year":"2001","publication_status":"published","citation":{"apa":"Rattenholl, A., Ruoppolo, M., Flagiello, A., Monti, M., Vinci, F., Marino, G., … Rudolph, R. (2001). Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor. <i>Journal of Molecular Biology</i>, <i>305</i>(3), 523–533. <a href=\"https://doi.org/10.1006/jmbi.2000.4295\">https://doi.org/10.1006/jmbi.2000.4295</a>","ama":"Rattenholl A, Ruoppolo M, Flagiello A, et al. Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor. <i>Journal of Molecular Biology</i>. 2001;305(3):523-533. doi:<a href=\"https://doi.org/10.1006/jmbi.2000.4295\">10.1006/jmbi.2000.4295</a>","ieee":"A. Rattenholl <i>et al.</i>, “Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor,” <i>Journal of Molecular Biology</i>, vol. 305, no. 3, pp. 523–533, 2001.","bibtex":"@article{Rattenholl_Ruoppolo_Flagiello_Monti_Vinci_Marino_Lilie_Schwarz_Rudolph_2001, title={Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor}, volume={305}, DOI={<a href=\"https://doi.org/10.1006/jmbi.2000.4295\">10.1006/jmbi.2000.4295</a>}, number={3}, journal={Journal of Molecular Biology}, publisher={Elsevier BV}, author={Rattenholl, Anke and Ruoppolo, Margherita and Flagiello, Angela and Monti, Maria and Vinci, Floriana and Marino, Gennaro and Lilie, Hauke and Schwarz, Elisabeth and Rudolph, Rainer}, year={2001}, pages={523–533} }","mla":"Rattenholl, Anke, et al. “Pro-Sequence Assisted Folding and Disulfide Bond Formation of Human Nerve Growth Factor.” <i>Journal of Molecular Biology</i>, vol. 305, no. 3, Elsevier BV, 2001, pp. 523–33, doi:<a href=\"https://doi.org/10.1006/jmbi.2000.4295\">10.1006/jmbi.2000.4295</a>.","short":"A. Rattenholl, M. Ruoppolo, A. Flagiello, M. Monti, F. Vinci, G. Marino, H. Lilie, E. Schwarz, R. Rudolph, Journal of Molecular Biology 305 (2001) 523–533.","alphadin":"<span style=\"font-variant:small-caps;\"><span style=\"font-variant:small-caps;\">Rattenholl, Anke</span> ; <span style=\"font-variant:small-caps;\">Ruoppolo, Margherita</span> ; <span style=\"font-variant:small-caps;\">Flagiello, Angela</span> ; <span style=\"font-variant:small-caps;\">Monti, Maria</span> ; <span style=\"font-variant:small-caps;\">Vinci, Floriana</span> ; <span style=\"font-variant:small-caps;\">Marino, Gennaro</span> ; <span style=\"font-variant:small-caps;\">Lilie, Hauke</span> ; <span style=\"font-variant:small-caps;\">Schwarz, Elisabeth</span> ; u. a.</span>: Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor. In: <i>Journal of Molecular Biology</i> Bd. 305, Elsevier BV (2001), Nr. 3, S. 523–533","chicago":"Rattenholl, Anke, Margherita Ruoppolo, Angela Flagiello, Maria Monti, Floriana Vinci, Gennaro Marino, Hauke Lilie, Elisabeth Schwarz, and Rainer Rudolph. “Pro-Sequence Assisted Folding and Disulfide Bond Formation of Human Nerve Growth Factor.” <i>Journal of Molecular Biology</i> 305, no. 3 (2001): 523–33. <a href=\"https://doi.org/10.1006/jmbi.2000.4295\">https://doi.org/10.1006/jmbi.2000.4295</a>."},"abstract":[{"lang":"eng","text":"Nerve growth factor (NGF) is a member of the neurotrophin family. These growth factors support neuronal survival and differentiation. Neurotrophins are synthesized as pre-pro-proteins. Whereas the pre-sequences mediate secretion, the function of the pro-peptides is largely unknown. To test the role of the pro-sequence as a folding enhancer, recombinant human pro-NGF (rh-pro-NGF) was produced in Escherichia coli. The oxidative refolding of rh-pro-NGF and rh-NGF was studied using electrospray mass spectrometry (ESIMS) time-course analysis. This analysis permitted both the identification and quantification of intermediates present during the process. The disulfide bonds formed at different times of the refolding processes were characterized by proteolytic digestion followed by matrix assisted laser desorption ionization mass spectrometry (MALDIMS) analysis. Folding yields and kinetics of rh-pro-NGF were significantly enhanced when compared to the in vitro refolding of mature rh-NGF. These results suggest that the pro-sequence of NGF promotes folding of the mature part."}],"alternative_id":["5384"],"volume":305,"language":[{"iso":"eng"}]}