The mature part of proNGF induces the structure of its pro‐peptide
M. Kliemannel, A. Rattenholl, R. Golbik, J. Balbach, H. Lilie, R. Rudolph, E. Schwarz, FEBS Letters 566 (2004) 207–212.
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Autor*in
Kliemannel, Marco;
Rattenholl, Anke
;
Golbik, Ralph;
Balbach, Jochen;
Lilie, Hauke;
Rudolph, Rainer;
Schwarz, Elisabeth
;
Golbik, Ralph;
Balbach, Jochen;
Lilie, Hauke;
Rudolph, Rainer;
Schwarz, ElisabethAbstract
Human nerve growth factor (NGF) belongs to the structural family of cystine knot proteins, characterized by a disulfide pattern in which one disulfide bond threads through a ring formed by a pair of two other disulfides connecting two adjacent β‐strands. Oxidative folding of NGF revealed that the pro‐peptide of NGF stimulates in vitro structure formation. In order to learn more about this folding assisting protein fragment, a biophysical analysis of the pro‐peptide structure has been performed. While proNGF is a non‐covalent homodimer, the isolated pro‐peptide is monomeric. No tertiary contacts stabilize the pro‐peptide in its isolated form. In contrast, the pro‐peptide appears to be structured when bound to the mature part. The results presented here demonstrate that the mature part stabilizes the structure in the pro‐peptide region. This is the first report that provides a biophysical analysis of a pro‐peptide of the cystine knot protein family.
Erscheinungsjahr
Zeitschriftentitel
FEBS Letters
Band
566
Zeitschriftennummer
1-3
Seite
207-212
ISSN
eISSN
FH-PUB-ID
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Kliemannel, Marco ; Rattenholl, Anke ; Golbik, Ralph ; Balbach, Jochen ; Lilie, Hauke ; Rudolph, Rainer ; Schwarz, Elisabeth: The mature part of proNGF induces the structure of its pro‐peptide. In: FEBS Letters Bd. 566, Wiley (2004), Nr. 1–3, S. 207–212
Kliemannel M, Rattenholl A, Golbik R, et al. The mature part of proNGF induces the structure of its pro‐peptide. FEBS Letters. 2004;566(1-3):207-212. doi:10.1016/j.febslet.2004.04.034
Kliemannel, M., Rattenholl, A., Golbik, R., Balbach, J., Lilie, H., Rudolph, R., & Schwarz, E. (2004). The mature part of proNGF induces the structure of its pro‐peptide. FEBS Letters, 566(1–3), 207–212. https://doi.org/10.1016/j.febslet.2004.04.034
@article{Kliemannel_Rattenholl_Golbik_Balbach_Lilie_Rudolph_Schwarz_2004, title={The mature part of proNGF induces the structure of its pro‐peptide}, volume={566}, DOI={10.1016/j.febslet.2004.04.034}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Kliemannel, Marco and Rattenholl, Anke and Golbik, Ralph and Balbach, Jochen and Lilie, Hauke and Rudolph, Rainer and Schwarz, Elisabeth}, year={2004}, pages={207–212} }
Kliemannel, Marco, Anke Rattenholl, Ralph Golbik, Jochen Balbach, Hauke Lilie, Rainer Rudolph, and Elisabeth Schwarz. “The Mature Part of ProNGF Induces the Structure of Its Pro‐peptide.” FEBS Letters 566, no. 1–3 (2004): 207–12. https://doi.org/10.1016/j.febslet.2004.04.034.
M. Kliemannel et al., “The mature part of proNGF induces the structure of its pro‐peptide,” FEBS Letters, vol. 566, no. 1–3, pp. 207–212, 2004.
Kliemannel, Marco, et al. “The Mature Part of ProNGF Induces the Structure of Its Pro‐peptide.” FEBS Letters, vol. 566, no. 1–3, Wiley, 2004, pp. 207–12, doi:10.1016/j.febslet.2004.04.034.
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