{"oa":"1","publisher":"Wiley","doi":"10.1002/biot.202100088","author":[{"first_name":"Mario","full_name":"Lederle, Mario","last_name":"Lederle"},{"last_name":"Tric","full_name":"Tric, Mircea","first_name":"Mircea"},{"first_name":"Tatjana","last_name":"Roth","full_name":"Roth, Tatjana"},{"first_name":"Lina","full_name":"Schütte, Lina","last_name":"Schütte"},{"full_name":"Rattenholl, Anke","last_name":"Rattenholl","orcid":"0009-0003-6828-5778","orcid_put_code_url":"https://api.orcid.org/v2.0/0009-0003-6828-5778/work/176557802","first_name":"Anke","id":"202382"},{"first_name":"Dirk","full_name":"Lütkemeyer, Dirk","last_name":"Lütkemeyer"},{"first_name":"Stefan","full_name":"Wölfl, Stefan","last_name":"Wölfl"},{"full_name":"Werner, Tobias","last_name":"Werner","first_name":"Tobias"},{"last_name":"Wiedemann","full_name":"Wiedemann, Philipp","first_name":"Philipp"}],"tmp":{"image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"intvolume":"        16","publication_identifier":{"eissn":["1860-7314"],"issn":["1860-6768"]},"date_created":"2025-01-23T15:14:34Z","date_updated":"2025-01-24T12:46:38Z","publication":"Biotechnology Journal","user_id":"220548","alternative_id":["5398"],"abstract":[{"text":"Great efforts are directed towards improving productivity, consistency and quality of biopharmaceutical processes and products. One particular area is the development of new sensors for continuous monitoring of critical bioprocess parameters by using online or in‐line monitoring systems. Recently, we developed a glucose biosensor applicable in single‐use, in‐line and long‐term glucose monitoring in mammalian cell bioreactors. Now, we integrated this sensor in an automated glucose monitoring and feeding system capable of maintaining stable glucose levels, even at very low concentrations. We compared this fed‐batch feedback system at both low (< 1 mM) and high (40 mM) glucose levels with traditional batch culture methods, focusing on glycosylation and glycation of the recombinant protein darbepoetin alfa (DPO) produced by a CHO cell line. We evaluated cell growth, metabolite and product concentration under different glucose feeding strategies and show that continuous feeding, even at low glucose levels, has no harmful effects on DPO quantity and quality. We conclude that our system is capable of tight glucose level control throughout extended bioprocesses and has the potential to improve performance where constant maintenance of glucose levels is critical.               ","lang":"eng"}],"language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1"}],"volume":16,"issue":"8","_id":"5369","article_number":"2100088","status":"public","type":"journal_article","year":"2021","publication_status":"published","title":"Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality","citation":{"ieee":"M. Lederle <i>et al.</i>, “Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality,” <i>Biotechnology Journal</i>, vol. 16, no. 8, 2021.","apa":"Lederle, M., Tric, M., Roth, T., Schütte, L., Rattenholl, A., Lütkemeyer, D., … Wiedemann, P. (2021). Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality. <i>Biotechnology Journal</i>, <i>16</i>(8). <a href=\"https://doi.org/10.1002/biot.202100088\">https://doi.org/10.1002/biot.202100088</a>","ama":"Lederle M, Tric M, Roth T, et al. Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality. <i>Biotechnology Journal</i>. 2021;16(8). doi:<a href=\"https://doi.org/10.1002/biot.202100088\">10.1002/biot.202100088</a>","alphadin":"<span style=\"font-variant:small-caps;\"><span style=\"font-variant:small-caps;\">Lederle, Mario</span> ; <span style=\"font-variant:small-caps;\">Tric, Mircea</span> ; <span style=\"font-variant:small-caps;\">Roth, Tatjana</span> ; <span style=\"font-variant:small-caps;\">Schütte, Lina</span> ; <span style=\"font-variant:small-caps;\">Rattenholl, Anke</span> ; <span style=\"font-variant:small-caps;\">Lütkemeyer, Dirk</span> ; <span style=\"font-variant:small-caps;\">Wölfl, Stefan</span> ; <span style=\"font-variant:small-caps;\">Werner, Tobias</span> ; u. a.</span>: Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality. In: <i>Biotechnology Journal</i> Bd. 16, Wiley (2021), Nr. 8","chicago":"Lederle, Mario, Mircea Tric, Tatjana Roth, Lina Schütte, Anke Rattenholl, Dirk Lütkemeyer, Stefan Wölfl, Tobias Werner, and Philipp Wiedemann. “Continuous Optical In‐line Glucose Monitoring and Control in CHO Cultures Contributes to Enhanced Metabolic Efficiency While Maintaining Darbepoetin Alfa Product Quality.” <i>Biotechnology Journal</i> 16, no. 8 (2021). <a href=\"https://doi.org/10.1002/biot.202100088\">https://doi.org/10.1002/biot.202100088</a>.","mla":"Lederle, Mario, et al. “Continuous Optical In‐line Glucose Monitoring and Control in CHO Cultures Contributes to Enhanced Metabolic Efficiency While Maintaining Darbepoetin Alfa Product Quality.” <i>Biotechnology Journal</i>, vol. 16, no. 8, 2100088, Wiley, 2021, doi:<a href=\"https://doi.org/10.1002/biot.202100088\">10.1002/biot.202100088</a>.","short":"M. Lederle, M. Tric, T. Roth, L. Schütte, A. Rattenholl, D. Lütkemeyer, S. Wölfl, T. Werner, P. Wiedemann, Biotechnology Journal 16 (2021).","bibtex":"@article{Lederle_Tric_Roth_Schütte_Rattenholl_Lütkemeyer_Wölfl_Werner_Wiedemann_2021, title={Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality}, volume={16}, DOI={<a href=\"https://doi.org/10.1002/biot.202100088\">10.1002/biot.202100088</a>}, number={82100088}, journal={Biotechnology Journal}, publisher={Wiley}, author={Lederle, Mario and Tric, Mircea and Roth, Tatjana and Schütte, Lina and Rattenholl, Anke and Lütkemeyer, Dirk and Wölfl, Stefan and Werner, Tobias and Wiedemann, Philipp}, year={2021} }"}}